osteoblast growth medium bulletkit (ogm)  (Lonza)

Journal: Materials

Article Title: Influence of Low Temperature Plasma Oxidizing on the Bioactivity of NiTi Shape Memory Alloy for Medical Applications

doi: 10.3390/ma16186086

Figure Lengend Snippet: Proliferation of osteoblasts cultured on NiTi in the initial state and with a TiO 2 surface layer represented as the number of cells per mm 2 and measured after 24 h, 48 h and 6 days of growth; * p ≤ 0.05 NiTi versus NiTi + TiO 2 .

Article Snippet: The cells were grown in an osteoblast basal medium enriched with fetal bovine serum (50 mL/500 mL medium), ascorbic acid (0.5 mL/500 mL medium), GA-1000 (0.5 mL/500 mL medium) antibiotics (OGM Osteoblast Growtth Medium BulletKit, Lonza), in a humid atmosphere of 95% air and 5% CO 2 at 37 °C.

Techniques: Cell Culture

Journal: Materials

Article Title: Influence of Low Temperature Plasma Oxidizing on the Bioactivity of NiTi Shape Memory Alloy for Medical Applications

doi: 10.3390/ma16186086

Figure Lengend Snippet: Morphology of osteoblasts cultured on the surfaces of NiTi alloy in the initial state ( a , a’ , c , c’ , e , e’ ) and with a TiO 2 surface layer ( b , b’ , d , d’ , f , f’ ) for 24 h ( a , a’ , b , b’ ), 48 h ( c , c’ , d , d’ ) and 6 days ( e , e’ , f , f’ ). SEM images magnified ×60 ( a – f ), ×500 ( a’ – f’ ).

Article Snippet: The cells were grown in an osteoblast basal medium enriched with fetal bovine serum (50 mL/500 mL medium), ascorbic acid (0.5 mL/500 mL medium), GA-1000 (0.5 mL/500 mL medium) antibiotics (OGM Osteoblast Growtth Medium BulletKit, Lonza), in a humid atmosphere of 95% air and 5% CO 2 at 37 °C.

Techniques: Cell Culture

Journal: Materials

Article Title: Hydroxyapatite from Natural Sources for Medical Applications

doi: 10.3390/ma15155091

Figure Lengend Snippet: Cell viability of osteoblasts for the 12 HAp samples. The stock solution was diluted in binary dilutions (1/2, 1/4, and 1/8).

Article Snippet: The 12 tested samples were placed in a 12-well cell culture plate, with growth medium completely specific to the osteoblast cell line (OGM TM Osteoblast Growth medium BulletKit TM , Lonza, Germany), and incubated for 24 h at 37 °C, in a shaking incubator, 5% CO 2 .

Techniques:

Journal: Materials

Article Title: Hydroxyapatite from Natural Sources for Medical Applications

doi: 10.3390/ma15155091

Figure Lengend Snippet: Cell proliferation of osteoblasts on HAp samples.

Article Snippet: The 12 tested samples were placed in a 12-well cell culture plate, with growth medium completely specific to the osteoblast cell line (OGM TM Osteoblast Growth medium BulletKit TM , Lonza, Germany), and incubated for 24 h at 37 °C, in a shaking incubator, 5% CO 2 .

Techniques:

Journal: Molecular cancer therapeutics

Article Title: The Cyclin-Dependent Kinase Inhibitor SCH 727965 (Dinacliclib) Induces the Apoptosis of Osteosarcoma Cells

doi: 10.1158/1535-7163.MCT-11-0167

Figure Lengend Snippet: SCH-induced apoptosis of newly prepared osteosarcoma cell lines and osteosarcoma tumor fragments but not of normal osteoblasts or fibroblasts. A, Osteo7770, Osteo8328, and Osteo8521 cells received SCH at the indicated concentrations for the indicated times. The percentage of apoptotic cells was determined by Annexin V binding. B, fragments of osteosarcoma xenografts grown in mice were cultured in medium containing the indicated concentrations of Rosc, Flav, or SCH for 30 hours. Cell extracts were Western blotted with antibody to PARP or actin. C, density-arrested NHOst, hFOB1.19, and WI-38 cells (gray bars) received the indicated concentrations of SCH for 72 hours. U2OS cells (black bars, positive control) received 20 nmol/L SCH for 72 hours. The percentage of apoptotic cells were determined by Annexin V binding. The 3 panels represent separate experiments. D, cells received the indicated amounts of SCH for 48 or 72 hours. The percentage of apoptotic cells was determined by Annexin V binding.

Article Snippet: Normal human osteoblasts (NHOst) were purchased from Lonza and were cultured in Osteoblast Growth Medium (OGM BulletKit, Lonza, Catalog # 3207).

Techniques: Binding Assay, Cell Culture, Western Blot, Positive Control